ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEADGLGCAV CVLTGASRGF GRALAPQLAR LLSPGSVMLV SARSESMLRQ LKEELGAQQP 

        70         80         90        100        110        120 
DLKVVLAAAD LGTEAGVQRL LSAVRELPRP EGLQRLLLIN NAATLGDVSK GFLNVNDLAE 

       130        140        150        160        170        180 
VNNYWALNLT SMLCLTSGTL NAFQDSPGLS KTVVNISSLC ALQPYKGWGL YCAGKAARDM 

       190        200        210        220        230        240 
LYQVLAAEEP SVRVLSYAPG PLDNDMQQLA RETSKDPELR SKLQKLKSDG ALVDCGTSAQ 

       250        260 
KLLGLLQKDT FQSGAHVDFY D 

References and documentation are available.

Please note the modified algorithm for extinction coefficient.

Number of amino acids: 261

Molecular weight: 27883.0

Theoretical pI: 5.56

Amino acid composition: 
Ala (A)  29	 11.1%
Arg (R)  13	  5.0%
Asn (N)  10	  3.8%
Asp (D)  15	  5.7%
Cys (C)   6	  2.3%
Gln (Q)  15	  5.7%
Glu (E)  12	  4.6%
Gly (G)  22	  8.4%
His (H)   1	  0.4%
Ile (I)   2	  0.8%
Leu (L)  45	 17.2%
Lys (K)  12	  4.6%
Met (M)   6	  2.3%
Phe (F)   5	  1.9%
Pro (P)  11	  4.2%
Ser (S)  21	  8.0%
Thr (T)  10	  3.8%
Trp (W)   2	  0.8%
Tyr (Y)   6	  2.3%
Val (V)  18	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1221
Hydrogen    H	      1993
Nitrogen    N	       341
Oxygen      O	       378
Sulfur      S	        12

Formula: C1221H1993N341O378S12
Total number of atoms: 3945

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20315
Abs 0.1% (=1 g/l)   0.729, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.715, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.14
This classifies the protein as stable.



Aliphatic index: 101.34

Grand average of hydropathicity (GRAVY): -0.008