enzyme kinetics

1. calculate Km and Vmax from the following data

[S] (uM)        vo (mM/sec)

0.1                0.34

0.2                0.53

0.4                0.74

0.8                0.91

1.6                1.04

 

2. determine the type of inhibition of an enzymatic reaction from the following data collected in the presence and absence of the inhibitor

[S] (mM)        vo (mM/min)            vo with inhibitor (mM/min)\

  1                    1.3                        0.8

  2                    2.0                        1.2

  4                    2.8                        1.7

  8                    3.6                        2.2

12                    4.0                        2.4