enzyme kinetics
1. calculate Km and Vmax from the following data
[S] (uM) vo (mM/sec)
0.1 0.34
0.2 0.53
0.4 0.74
0.8 0.91
1.6 1.04
2. determine the type of inhibition of an enzymatic reaction from the following data collected in the presence and absence of the inhibitor
[S] (mM) vo (mM/min) vo with inhibitor (mM/min)\
1 1.3 0.8
2 2.0 1.2
4 2.8 1.7
8 3.6 2.2
12 4.0 2.4